2HE4
The crystal structure of the second PDZ domain of human NHERF-2 (SLC9A3R2) interacting with a mode 1 PDZ binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97646 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 49.803, 37.677, 40.340 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 39.970 - 1.450 |
| R-factor | 0.13203 |
| Rwork | 0.130 |
| R-free | 0.18003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i92 and 1g9o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.594 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.970 | 1.550 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.038 | 0.140 |
| Number of reflections | 13060 | |
| <I/σ(I)> | 6.45 | |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 0.98 | 0.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
