2HDX
Crystal structure of the Src homology-2 domain of SH2-B in complex with Jak2 pTyr813 phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2005-05-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.210, 74.190, 239.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.350 |
R-factor | 0.24 |
Rwork | 0.210 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2HDV |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.350 | 2.000 |
Rmerge | 0.072 | |
Number of reflections | 33327 | |
<I/σ(I)> | 16.1 | 10.2 |
Completeness [%] | 98.1 | 97.6 |
Redundancy | 4.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 25% PEG 4000, 0.1 M sodium citrate, 0.3 M ammonium acetate., pH 5.6 |