2HDV
Crystal structure of the Src Homology-2 domain of the adapter protein SH2-B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2005-04-09 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.050, 57.870, 99.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rpy |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 14330 | |
<I/σ(I)> | 27.3 | 10.2 |
Completeness [%] | 99.2 | 97.6 |
Redundancy | 3.9 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 30% (w/v) PEG 4000, 0.1 M Tris HCl 605 0.2 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 8.50 |