2HC2
Engineered protein tyrosine phosphatase beta catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Detector technology | CCD |
Collection date | 2006-02-02 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.314, 70.775, 49.846 |
Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
Resolution | 33.670 - 1.400 |
R-factor | 0.14571 |
Rwork | 0.144 |
R-free | 0.17892 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hc1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.206 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.670 | 1.500 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.028 | 0.210 |
Number of reflections | 48397 | |
<I/σ(I)> | 20.2 | 4.87 |
Completeness [%] | 92.5 | 69.4 |
Redundancy | 2.6 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.85 | 298 | 21% PEG 8000, 200 mM MgCl2, 1% BME, 0.1% BOG, 1mM DTT, 80 mM Na Citrate, pH 7.85, VAPOR DIFFUSION, HANGING DROP, temperature 298K |