2HAP
STRUCTURE OF A HAP1-18/DNA COMPLEX REVEALS THAT PROTEIN/DNA INTERACTIONS CAN HAVE DIRECT ALLOSTERIC EFFECTS ON TRANSCRIPTIONAL ACTIVATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 133 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-06-15 |
| Detector | SIEMENS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.500, 92.500, 86.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.30200 |
| Structure solution method | MIR |
| RMSD bond length | 0.001 * |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Total number of observations | 30673 * | |
| Number of reflections | 16925 | |
| Completeness [%] | 96.9 | 62 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 20 * | 0.3 MM PROTEIN, 0.4 MM DNA DUPLEX, 5% PEG 2000, 100 MM KCL, 5 MM MGCL2, 0.1 MM CO(NH3)6CL3, 25 MM MES (PH 5.6), VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCL | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | [CO(NH3)6]CL3 | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | PEG 2000 | ||
| 6 | 1 | 2 | PEG 2000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.3 (mM) | |
| 10 | 1 | reservoir | 0.2 (mM) | ||
| 11 | 1 | reservoir | MES | 50 (mM) | |
| 2 | 1 | drop | DNA duplex | 0.4 (mM) | |
| 3 | 1 | drop | PEG | 5 (%) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | 0.1 (mM) | ||
| 7 | 1 | drop | MES | 25 (mM) | |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | 10 (mM) |
