2GZ3
Structure of Aspartate Semialdehyde Dehydrogenase (ASADH) from Streptococcus pneumoniae complexed with NADP and aspartate-semialdehyde
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-01-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 |
Unit cell lengths | 59.348, 64.439, 96.739 |
Unit cell angles | 89.98, 81.06, 82.00 |
Refinement procedure
Resolution | 30.010 - 2.100 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2GZ1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.334 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK (9.2SSI) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.580 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.113 | 0.293 |
Total number of observations | 18514 | |
Number of reflections | 95735 | |
<I/σ(I)> | 6.6 | 3.3 |
Completeness [%] | 92.7 | 89.6 |
Redundancy | 3.3 | 3.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 50 mM Citrate pH 6.0 200 mM ammonium acetate 20% PEG 8000 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |