2GYS
2.7 A structure of the extracellular domains of the human beta common receptor involved in IL-3, IL-5, and GM-CSF signalling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.117 |
| Spacegroup name | H 3 |
| Unit cell lengths | 184.909, 184.909, 101.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.21426 |
| Rwork | 0.212 |
| R-free | 0.26913 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1gh7 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.060 | 0.153 |
| Number of reflections | 32327 | |
| <I/σ(I)> | 19.2 | 2.4 |
| Completeness [%] | 91.4 | 40.2 |
| Redundancy | 5.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 277 | 10% polyethylene glycol 5000 mono-methyl ether in phosphate buffer, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
