2GUI
Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. coli DNA Polymerase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.128 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.099, 60.099, 109.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.880 - 1.600 |
| R-factor | 0.16195 |
| Rwork | 0.160 |
| R-free | 0.19746 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1j53 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.372 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.900 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.058 | 0.155 |
| Number of reflections | 27900 | |
| <I/σ(I)> | 9 | 4.97 |
| Completeness [%] | 99.6 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 0.1 M Cacodylate Acid, 20% PEG-8K, 2.5 mM UMP, 5 mM MnSO4, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
