2GNN

Crystal Structure of the Orf Virus NZ2 Variant of VEGF-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-27
Detector	MAR CCD 165 mm
Wavelength(s)	1.00017
Spacegroup name	P 41 2 2
Unit cell lengths	98.600, 98.600, 240.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.480 - 2.300
R-factor	0.224
Rwork	0.223
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.250
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.081	0.799
Number of reflections	52820	5379
<I/σ(I)>	14.58	3.1
Completeness [%]	93.4	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.6 M Ammonium sulfate, 3% PEG 4K, 0.1 M Sodium citrate, 0.3% Benzamidine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.6 M Ammonium sulfate, 3% PEG 4K, 0.1 M Sodium citrate, 0.3% Benzamidine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.6 M Ammonium sulfate, 3% PEG 4K, 0.1 M Sodium citrate, 0.3% Benzamidine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K