2GIF
Asymmetric structure of trimeric AcrB from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 222.800, 134.100, 161.010 |
| Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
| Resolution | 29.510 - 2.900 |
| R-factor | 0.22761 |
| Rwork | 0.226 |
| R-free | 0.26677 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.073 | 0.956 |
| Number of reflections | 103134 | |
| <I/σ(I)> | 13.4 | 1.6 |
| Completeness [%] | 99.3 | 97 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 290 | 5% PEG 400, 16-22% PEG 300, 8-11% glycerol, 70mM sodium citrate, pH 4.6, VAPOR DIFFUSION, temperature 290K |
