2GF9

Crystal structure of human RAB3D in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-03-13
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	35.700, 63.222, 67.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.250 - 1.530
Rwork	0.153
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rab
RMSD bond length	0.015
RMSD bond angle	1.344
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.250	30.000	1.580
High resolution limit [Å]	1.530	3.290	1.530
Rmerge	0.035	0.030	0.056
Number of reflections	21079	2536	659
<I/σ(I)>	32.8
Completeness [%]	88.3
Redundancy	7.4	8.5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	20% PEG8000, 0.2M magnesium acetate, 0.1M sodium cacodylate, pH 6.5, vapor diffusion, sitting drop, temperature 291K