2G1M
Cellular Oxygen Sensing: Crystal Structure of Hypoxia-Inducible Factor Prolyl Hydroxylase (PHD2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-10 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 63 |
| Unit cell lengths | 110.742, 110.742, 39.986 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.710 - 2.200 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.28900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | SADABS |
| Data scaling software | SAINT |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.370 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.239 | |
| Number of reflections | 27841 | |
| <I/σ(I)> | 31.2 | 3.25 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 45.9 | 40 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 1.6M AMMONIUM SULFATE, 4% DIOXANE, 0.1M MES PH6.5, ARGON ATMOSPHERE, 20MG/ML PROTEIN WITH 1MM FE(II)SO4, 2.0MM IIQ, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
