2G19
Cellular Oxygen Sensing: Crystal Structure of Hypoxia-Inducible Factor Prolyl Hydroxylase (PHD2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-12-14 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 63 |
Unit cell lengths | 110.851, 110.851, 40.379 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.000 - 1.700 |
Rwork | 0.216 |
R-free | 0.25300 |
Structure solution method | COMBINED MAD AND SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.513 | |
Number of reflections | 30804 | |
<I/σ(I)> | 32 | 2 |
Completeness [%] | 97.9 | 93.6 |
Redundancy | 10 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.4 | 298 | 28% polyethylene glycol 8000, 0.2 M ammonium sulfate, 100 mM MES buffer with 10 fold molar excess of inhibitor, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 6.40 | |
1 | 6.4 | 298 | 28% polyethylene glycol 8000, 0.2 M ammonium sulfate, 100 mM MES buffer with 10 fold molar excess of inhibitor, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 6.40 |