2FTW
Crystal structure of dihydropyrimidinase from dictyostelium discoideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.939 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 84.600, 89.599, 134.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.050 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kcx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.182 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.459 | 2.160 |
| High resolution limit [Å] | 2.000 | 2.050 |
| Rmerge | 0.134 | 0.662 |
| Total number of observations | 12225 | |
| Number of reflections | 30568 | 4408 |
| <I/σ(I)> | 4.3 | 0.9 |
| Completeness [%] | 94.4 | 94.4 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG 1500, 0.1M MMT buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
