2FSZ
A second binding site for hydroxytamoxifen within the coactivator-binding groove of estrogen receptor beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 |
| Unit cell lengths | 105.040, 105.040, 102.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.110 - 2.200 |
| R-factor | 0.274 |
| Rwork | 0.273 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 27338 | |
| Completeness [%] | 95.7 | 67.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 0.1M sodium citrate, 1.5M sodium chloride, 4% ethylene imine, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
