2FIM
Structure of the C-terminal domain of Human Tubby-like protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.960, 85.150, 58.790 |
| Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
| Resolution | 19.890 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.239 |
| Number of reflections | 43277 | 7645 |
| <I/σ(I)> | 15.9 | 5.5 |
| Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M AS, 25% PEG 4000, 15% glycerol, 50mM 3-(N,N-Dimethyloctylammonio)propanesulfonate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
