2FIF
Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2820 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.200, 44.420, 69.170 |
| Unit cell angles | 90.00, 108.98, 90.00 |
Refinement procedure
| Resolution | 90.910 - 2.490 |
| R-factor | 0.23003 |
| Rwork | 0.228 |
| R-free | 0.26347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fid |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.991 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.910 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 19257 | |
| Completeness [%] | 98.8 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 20-25% PEG 4000, 0.2M lithium sulfate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
