2FHJ
Crystal structure of formylmethanofuran: tetrahydromethanopterin formyltransferase in complex with its coenzymes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-04-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.000, 74.150, 103.870 |
| Unit cell angles | 90.00, 113.54, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1FTR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.613 |
| Data reduction software | DENZO |
| Data scaling software | XSCALE |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.126 | 0.502 |
| Number of reflections | 75995 | 9923 |
| <I/σ(I)> | 7.93 | 3.38 |
| Completeness [%] | 94.7 | 91.7 |
| Redundancy | 3 | 2.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12-18 % PEG 8000, 20 % glycerol, 0.5 M KCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
