2FF6
Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.221, 69.373, 79.650 |
| Unit cell angles | 90.00, 95.10, 90.00 |
Refinement procedure
| Resolution | 29.500 - 2.050 |
| R-factor | 0.20406 |
| Rwork | 0.202 |
| R-free | 0.23679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.076 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.310 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 36145 | |
| Completeness [%] | 99.0 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 7% PEG 3000, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
