2FDB
Crystal Structure of Fibroblast growth factor (FGF)8b in complex with FGF Receptor (FGFR) 2c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2004-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.846, 46.908, 109.616 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.280 |
| R-factor | 0.23931 |
| Rwork | 0.239 |
| R-free | 0.27190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1evt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 44825 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18.5% mPEG 5000, 0.15M magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
