2FCC
Crystal Structure of T4 Pyrimidine Dimer Glycosylase (T4-Pdg) Covalently Complexed with a DNA Substrate Containing Abasic Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9188 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 184.059, 184.059, 100.855 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.850 - 2.300 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vas |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.393 |
| Number of reflections | 81403 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 2 M (NH4)2SO4 and 0.1 M sodium citrate buffer (pH 6.4), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
