2F9O

Crystal Structure of the Recombinant Human Alpha I Tryptase Mutant D216G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-11-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	83.310, 88.930, 162.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.900 - 2.100
R-factor	0.216
Rwork	0.216
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f9n
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.900	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.071	0.339
Number of reflections	70731
<I/σ(I)>	18.7
Completeness [%]	99.7	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	27.5% PEG 1500, pH 6.80, VAPOR DIFFUSION, SITTING DROP, temperature 293K