2F9G

Crystal structure of Fus3 phosphorylated on Tyr182

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-23
Detector	ADSC QUANTUM 4
Wavelength(s)	1.115889
Spacegroup name	P 21 21 21
Unit cell lengths	56.811, 62.533, 86.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.211
Rwork	0.211
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b9f
RMSD bond length	0.013
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	18096
<I/σ(I)>	10.7	2.2
Completeness [%]	97.3	86.6
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	25-28% PEG1000, 0.1M MES, 5-10% MPD, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K