2F9G

Crystal structure of Fus3 phosphorylated on Tyr182

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000749	biological_process	response to pheromone triggering conjugation with cellular fusion
A	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
A	0001403	biological_process	invasive growth in response to glucose limitation
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004707	molecular_function	MAP kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0006468	biological_process	protein phosphorylation
A	0010494	cellular_component	cytoplasmic stress granule
A	0010526	biological_process	retrotransposon silencing
A	0016310	biological_process	phosphorylation
A	0035556	biological_process	intracellular signal transduction
A	0042597	cellular_component	periplasmic space
A	0042802	molecular_function	identical protein binding
A	0043332	cellular_component	mating projection tip
A	0043409	biological_process	negative regulation of MAPK cascade
A	0046827	biological_process	positive regulation of protein export from nucleus
A	0051301	biological_process	cell division
A	0071507	biological_process	pheromone response MAPK cascade
A	0106310	molecular_function	protein serine kinase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 600

Chain	Residue
A	ASN142
A	ASP155
A	ADP500
A	HOH699
A	HOH700

---

site_id	AC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ADP A 500

Chain	Residue
A	LYS42
A	ARG55
A	GLN93
A	GLU94
A	MET96
A	ASP99
A	ARG102
A	SER141
A	ASN142
A	LEU144
A	ASP155
A	MG600
A	HOH631
A	HOH638
A	HOH670
A	HOH699
A	HOH700
A	LEU19
A	TYR24
A	GLY25
A	VAL27

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	25
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGEGAYGVVCsAthkptgeiv.........AIKK

Chain	Residue	Details
A	LEU19-LYS43

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ViHrDLKpsNLLI

Chain	Residue	Details
A	VAL133-ILE145

---

site_id	PS01351
Number of Residues	103
Details	MAPK MAP kinase signature. FdkplfalrtlREikilkhfkheniitifniqrpdsfenfnevyiiqelmqtdlhrvistqmlsddhiqyfiyqtlravkvlhgsnvih..........RDlKpsnllinsnC

Chain	Residue	Details
A	PHE47-CYS149

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027

Chain	Residue	Details
A	ASP137

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159

Chain	Residue	Details
A	LEU19
A	LYS42

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:1628831

Chain	Residue	Details
A	THR180

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:1628831

Chain	Residue	Details
A	PTR182

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
A	LYS345

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	SER141
A	ASP137

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	LYS139
A	ASP137

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	THR185
A	LYS139
A	ASP137

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASN142
A	LYS139
A	ASP137

---