2B9F

Crystal structure of non-phosphorylated Fus3

Summary for 2B9F

• Entry DOI: 10.2210/pdb2b9f/pdb
• Related: 2B9H 2B9I 2B9J
• Descriptor: Mitogen-activated protein kinase FUS3, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: transferase
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Nucleus: P16892
• Total number of polymer chains: 1
• Total formula weight: 41278.53

Authors

Remenyi, A.,Good, M.C.,Bhattacharyya, R.P.,Lim, W.A. (deposition date: 2005-10-11, release date: 2006-01-03, Last modification date: 2023-08-23)

Primary citation

Remenyi, A.,Good, M.C.,Bhattacharyya, R.P.,Lim, W.A.
The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network.
Mol.Cell, 20:951-962, 2005

PubMed Abstract: Cells use a network of mitogen-activated protein kinases (MAPKs) to coordinate responses to diverse extracellular signals. Here, we examine the role of docking interactions in determining connectivity of the yeast MAPKs Fus3 and Kss1. These closely related kinases are activated by the common upstream MAPK kinase Ste7 yet generate distinct output responses, mating and filamentous growth, respectively. We find that docking interactions are necessary for communication with the kinases and that they can encode subtle differences in pathway-specific input and output. The cell cycle arrest mediator Far1, a mating-specific substrate, has a docking motif that selectively binds Fus3. In contrast, the shared partner Ste7 has a promiscuous motif that binds both Fus3 and Kss1. Structural analysis reveals that Fus3 interacts with specific and promiscuous peptides in conformationally distinct modes. Induced fit recognition may allow docking peptides to achieve discrimination by exploiting subtle differences in kinase flexibility.

PubMed: 16364919
DOI: 10.1016/j.molcel.2005.10.030

Experimental method

X-RAY DIFFRACTION (1.8 Å)