2F6J
Crystal structure of PHD finger-linker-bromodomain fragment of human BPTF in the H3(1-15)K4me3 bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.3477 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.579, 84.987, 109.385 |
Unit cell angles | 90.00, 99.23, 90.00 |
Refinement procedure
Resolution | ? - 2.000 |
Rwork | 0.198 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.105 | 0.459 |
Number of reflections | 46877 | |
<I/σ(I)> | 15 | 4.2 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 17% PEG 4000, 8.5% iso-Propanol, 0.085M Hepes-Na pH 7.5, 15% Glycerol anhydrous, VAPOR DIFFUSION, HANGING DROP, temperature 293K |