2F64
Crystal structure of Nucleoside 2-deoxyribosyltransferase from Trypanosoma brucei at 1.6 A resolution with 1-METHYLQUINOLIN-2(1H)-ONE bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92014 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.827, 75.351, 87.048 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1663 |
| Rwork | 0.165 |
| R-free | 0.18712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a0k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.934 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.686 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 46217 | |
| <I/σ(I)> | 11 | 2.2 |
| Completeness [%] | 95.6 | 92.53 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 30 % PEG MME 2000, 0.2 ammonium sulfate, 0.1 sodium acetate trihydrate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
