2F36
Crystal Structure of the GluR5 Ligand Binding Core Dimer with Glutamate At 2.1 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.694, 73.784, 115.918 |
| Unit cell angles | 90.00, 99.74, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.110 |
| R-factor | 0.19124 |
| Rwork | 0.186 |
| R-free | 0.24111 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1TXf |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.839 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.344 |
| Number of reflections | 53310 | |
| <I/σ(I)> | 12.3 | 3.62 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 1.75 M Ammonium Sulfate 100 mM HEPES 10 mM glutamic acid, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
