2F2T
Crystal structure of Nucleoside 2-deoxyribosyltransferase from Trypanosoma brucei at 1.7 A resolution with 5-Aminoisoquinoline bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92020 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.960, 75.493, 86.324 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.17794 |
| Rwork | 0.177 |
| R-free | 0.20373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a0k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.863 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.792 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 36740 | |
| <I/σ(I)> | 10.7 | 3.9 |
| Completeness [%] | 92.8 | 67.24 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 30 % PEG MME 2000 0.2 ammonium sulfate 0.1 sodium acetate trihydrate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
