2F16
Crystal structure of the yeast 20S proteasome in complex with bortezomib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 136.175, 300.716, 144.659 |
Unit cell angles | 90.00, 113.28, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yrp |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.091 | |
Number of reflections | 249075 | |
Completeness [%] | 96.5 | 80.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 12 % MPD 28mM MgAc2 50mM MES-NaOH, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |