2EZ6
Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-02 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.839, 50.976, 113.746 |
| Unit cell angles | 90.00, 104.47, 90.00 |
Refinement procedure
| Resolution | 29.840 - 2.050 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1RC7 1yz9 1yyw 1yyo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.061 | 0.411 |
| Number of reflections | 40208 | |
| <I/σ(I)> | 13.3 | 1.6 |
| Completeness [%] | 90.6 | 55.2 |
| Redundancy | 2.5 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M ammonium sulfate, 25% w/v PEG 3350 in 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | ammonium sulfate | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | H2O |
