1YYO

Crystal structure of RNase III mutant E110K from Aquifex aeolicus complexed with double-stranded RNA at 2.9-Angstrom Resolution

Summary for 1YYO

• Entry DOI: 10.2210/pdb1yyo/pdb
• Related: 1I4S 1JFZ 1O0W 1RC5 1RC7 1YYK 1YYW 1YZ9
• Descriptor: 5'-R(*CP*GP*CP*GP*AP*AP*UP*UP*CP*GP*CP*G)-3', Ribonuclease III, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• Functional Keywords: ribonuclease iii, double-stranded rna, rna interference, endonucleolytic cleavage, hydrolase-rna complex, hydrolase/rna
• Biological source: Aquifex aeolicus
• Cellular location: Cytoplasm (By similarity): O67082
• Total number of polymer chains: 6
• Total formula weight: 67726.38

Authors

Gan, J.,Tropea, J.E.,Austin, B.P.,Court, D.L.,Waugh, D.S.,Ji, X. (deposition date: 2005-02-25, release date: 2005-11-22, Last modification date: 2023-08-30)

Primary citation

Gan, J.,Tropea, J.E.,Austin, B.P.,Court, D.L.,Waugh, D.S.,Ji, X.
Intermediate states of ribonuclease III in complex with double-stranded RNA
Structure, 13:1435-1442, 2005

PubMed Abstract: Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates.

PubMed: 16216575
DOI: 10.1016/j.str.2005.06.014

Experimental method

X-RAY DIFFRACTION (2.9 Å)