2ESV

Structure of the HLA-E-VMAPRTLIL/KK50.4 TCR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	235.096, 41.462, 112.471
Unit cell angles	90.00, 114.66, 90.00

Refinement procedure

Resolution	36.400 - 2.600
R-factor	0.218
Rwork	0.214
R-free	0.29200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.016
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.600	2.600
Rmerge		0.104
Number of reflections	67491
<I/σ(I)>		11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	19-22% PEG 3350, 0.2M Potassium Iodide, pH 6.9-7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K