2ESL
Human Cyclophilin C in Complex with Cyclosporin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | SBC-3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.203, 123.802, 135.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.400 - 1.900 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.373 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.515 |
| Number of reflections | 95283 | |
| <I/σ(I)> | 25.5 | 3.68 |
| Completeness [%] | 98.6 | 97.8 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG550 MME, 10MM ZINC ACETATE, 100MM MES, PH 6.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298.0K |
