2EC0
RNA-dependent RNA polymerase of foot-and-mouth disease virus in complex with a template-primer RNA and ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 197 |
Detector technology | CCD |
Collection date | 2004-11-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 95.656, 95.656, 201.458 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.750 |
R-factor | 0.24741 |
Rwork | 0.245 |
R-free | 0.29548 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u09 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.075 |
Number of reflections | 28495 |
<I/σ(I)> | 7.6 |
Completeness [%] | 95.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 30% PEG 4000, 0.2M magnesium acetate, 0.1M sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | sodium cacodylate | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | PEG 4000 | ||
6 | 1 | 2 | magnesium acetate | ||
7 | 1 | 2 | sodium cacodylate | ||
8 | 1 | 2 | HOH |