2EB1

Crystal Structure of the C-Terminal RNase III Domain of Human Dicer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2005-06-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	88.602, 199.743, 119.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.212
Rwork	0.211
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jfz
RMSD bond length	0.020
RMSD bond angle	1.483
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	72223

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	278	1.0M ammonium sulfate, 0.1M TRIS-HCL, 0.01M MGCL2, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 278K