2DVS
Crystal structure analysis of the N-terminal bromodomain of human BRD2 complexed with acetylated histone H4 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-07-07 |
Detector | ADSC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.149, 55.248, 67.364 |
Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.040 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BRD2-BD1 NATIVE |
RMSD bond length | 0.017 |
RMSD bond angle | 1.413 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.057 | 0.188 |
Number of reflections | 26788 | |
Completeness [%] | 99.5 | 98.9 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, CO-CRYSTALLIZAATION | 6.5 | 298 | PEGMME 5K, AS, pH 6.50, VAPOR DIFFUSION, CO-CRYSTALLIZAATION, temperature 298K |