2DSP
Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.330, 38.990, 61.330 |
| Unit cell angles | 90.00, 99.89, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22269 |
| Rwork | 0.220 |
| R-free | 0.27094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wqj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 5177 | |
| Completeness [%] | 96.0 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 23% PEG 1500, 25mM Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
