2DRD
Crystal structure of a multidrug transporter reveal a functionally rotating mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-13 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 225.801, 134.466, 162.115 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.100 |
| R-factor | 0.257 |
| Rwork | 0.255 |
| R-free | 0.31000 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.784 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | FFTBIG |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.085 | 0.306 |
| Number of reflections | 84986 | |
| <I/σ(I)> | 17.6 | 2.6 |
| Completeness [%] | 97.9 | 96.8 |
| Redundancy | 9.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | PEG4000, PH 6.1, VAPOR DIFFUSION, SITTING DROP |
