2DP6
Crystal structure of uracil-DNA glycosylase in complex with AP:C containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.868, 148.754, 93.086 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.250 - 1.800 |
Rwork | 0.204 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ddg |
RMSD bond length | 0.005 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.296 |
Number of reflections | 44735 | |
<I/σ(I)> | 35.6 | 6.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 293 | 1.4M NaKPO4, pH 3.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaKPO4 | ||
2 | 1 | 1 | HOH | ||
3 | 1 | 2 | NaKPO4 | ||
4 | 1 | 2 | HOH |