2D39
Trivalent Recognition Unit of Innate Immunity System; Crystal Structure of human M-ficolin Fibrinogen-like Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-07 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.82656 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.160, 117.450, 55.190 |
| Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.209 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 53715 |
| Completeness [%] | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 17% PEG 4000,320mM Li2SO4, 100mM MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
