2CO0
WDR5 and unmodified Histone H3 complex at 2.25 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.916, 47.030, 101.768 |
| Unit cell angles | 90.00, 107.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1erj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.150 | 0.570 |
| Number of reflections | 28460 | |
| <I/σ(I)> | 15.8 | 3.07 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
