2CN0
Complex of Recombinant Human Thrombin with a Designed Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.640, 71.410, 72.520 |
| Unit cell angles | 90.00, 100.35, 90.00 |
Refinement procedure
| Resolution | 71.430 - 1.300 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2CF8.PDB |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 83900 | |
| <I/σ(I)> | 14.2 | 5.1 |
| Completeness [%] | 96.4 | 100 |
| Redundancy | 4.67 | 4.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | PH 7.40 |
