2CMO

The structure of a mixed glur2 ligand-binding core dimer in complex with (s)-glutamate and the antagonist (s)-ns1209

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	CCD
Collection date	2002-09-26
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	62.554, 92.953, 96.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.060 - 2.650
R-factor	0.215
Rwork	0.215
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1FTL AND 1FTJ
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.060	2.740
High resolution limit [Å]	2.650	2.650
Rmerge	0.100	0.350
Number of reflections	15865
<I/σ(I)>	6	2
Completeness [%]	88.8	87.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.50