2CL0

CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-18
Detector	ADSC CCD
Spacegroup name	H 3 2
Unit cell lengths	88.540, 88.540, 144.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.420 - 1.800
R-factor	0.148
Rwork	0.146
R-free	0.18500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5p21
RMSD bond length	0.016
RMSD bond angle	1.815
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.420	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.080	0.880
Number of reflections	23933
<I/σ(I)>	16.9	2.05
Completeness [%]	99.9	88.8
Redundancy	11.6	6.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6		RESERVOIR SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0.1 MM SODIUM AZIDE, 26% PEG 400; PROTEIN SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0.1 MM SODIUM AZIDE