2CG5
Structure of aminoadipate-semialdehyde dehydrogenase- phosphopantetheinyl transferase in complex with cytosolic acyl carrier protein and coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-12 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.361, 69.361, 184.715 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2byd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.580 |
| Number of reflections | 14864 | |
| <I/σ(I)> | 17.9 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.4 M (NH4)H2PO4 |
