2C78
EF-Tu complexed with a GTP analog and the antibiotic pulvomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-30 |
| Detector | MARRESERCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.686, 86.686, 105.514 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 1.400 |
| R-factor | 0.1967 |
| Rwork | 0.197 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eft |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 88023 | |
| <I/σ(I)> | 39.4 | 2.5 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 4.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 18-23% PEG6000, 3-5% GLYCEROL, pH 7.00 |
