2C54
gdp-mannose-3', 5' -epimerase (arabidopsis thaliana),k178r, with gdp-beta-l-gulose and gdp-4-keto-beta-l-gulose bound in active site.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-13 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.278, 82.457, 65.875 |
Unit cell angles | 90.00, 98.77, 90.00 |
Refinement procedure
Resolution | 64.550 - 1.500 |
R-factor | 0.121 |
Rwork | 0.118 |
R-free | 0.16700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.943 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.300 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.050 | 0.210 |
Number of reflections | 105071 | |
<I/σ(I)> | 9.6 | 3.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | PROTEIN CRYSTALISED IN 100MM HEPES PH 7.4, 2.16 M AMMONIUM SULPHATE, VAPOUR DIFFUSION, SITTING DROP. CRYOPROTECTED WITH 4M SODIUM FORMATE. |