2C4R

Catalytic domain of E. coli RNase E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS
Synchrotron site	ALS
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 62 2 2
Unit cell lengths	196.586, 196.586, 140.766
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 3.600
R-factor	0.32
Rwork	0.319
R-free	0.34700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bx2
RMSD bond length	0.016
RMSD bond angle	1.975
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.130	0.640
Number of reflections	19065
<I/σ(I)>	23	209
Completeness [%]	99.8	97.7
Redundancy	16	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		CRYSTALLIZATION CONDITIONS: CRYSTALS OF THE RNASE E CATALYTIC DOMAIN/ RNA COMPLEX APPEARED AFTER TWO TO FOUR WEEKS IN 5 TO 20 % WT/V POLYETHYLENE GLYCOL 8,000, 0.1 M TRIS PH 7.5 TO 8.0, AND 10 TO 50 MM MAGNESIUM FORMATE AT 20OC