2BYT

Thermus thermophilus Leucyl-tRNA synthetase complexed with a tRNAleu transcript in the post-editing conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-16
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	203.869, 125.578, 172.979
Unit cell angles	90.00, 118.44, 90.00

Refinement procedure

Resolution	24.450 - 3.300
R-factor	0.243
Rwork	0.243
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h3n
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	4.000
High resolution limit [Å]	3.300	3.300
Rmerge	0.100	0.220
Number of reflections	57205
<I/σ(I)>	12.1	7.33
Completeness [%]	98.7	98.4
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		5MG/ML LEUCYL-TRNA SYNTHETASE MOLAR RATIO PROTEIN:TRNA 1.0:1.2 5 MM L-LEUCINE 15MM MGCL2 50MM MES PH6.5 0.8M AMMONIUM SULPHATE AGAINST RESERVOIR CONTAINING 1.5M AMMONIUM SULPHATE AND 0.1M MES PH6.5, pH 7.50